Volume (5), Issue (2), (2019):
Balay-odao, E.M., Marbella, I.M.V., Balisong, J. (2019) Level of Depression and Suicidal Ideation among BSN Students. Journal of basic and applied Research in Biomedicine, 5(2): 73-80
Abstract:Undergraduate college students have been identified as at-risk population for development of suicidal ideation and depression. Student nurses are among those who experience depression and anxiety due to stressful life events. The stressful situations encountered by student nurses are multifaceted. Thus, this study explored the level of depression and suicidal ideation of BSN students and the effect of gender and year level. The need to explore these two salient factors was prompted by the need to promote and to improve school of nursing services, student safety, and decrease cases of depression and suicidal ideation among students. The study utilized a descriptive design. There were 326 student nurses who participated in the study. The questionnaire which was used for this study was based on 2 standardized self – report questionnaire. The findings showed that most of the students experienced mild to severe depression. Most of the students however, do not have suicidal ideation behavior, but almost one third of the students have a high risk for suicidal ideation behavior. Furthermore, the findings showed that gender and year level have no significant effect on the level of depression among BSN Students. Gender and year level have no significant effects in the level of suicidal ideation among student nurses. These findings can help guide the development of a school-based, prevention and promotion mental health program.
Qaralleh, H., Al-limoun, M.O., Khleifat, K.M, Alsharafa, K.Y., Al Tarawneh, A.A. (2019). Vitreoscilla sp. hemoglobin (VHb) Expression in Heterologous Hosts: Some Aspects of Their Biotechnological Application. Journal of basic and applied Research in Biomedicine, 5(2): 81-88
Abstract:It is worth mentioning that the high output of different physiological responses under the expression of vgb, may have a considerable effect on the enzyme productivity, dairy industry, heavy metal uptake, biodegradation of different organic pollutants and other applications. The expression of bacterial haemoglobin is useful in lessening the load of perceived toxic conditions such as high oxygen levels. This in turn probably has the same impact on some peripheral toxic materials. This, hemoglobin biotechnology can be extended to enhance production of pollutants degrading enzymes or production of some valuable manufacturing materials on the case-by-case bases. It is likely that the mechanism of bacterial hemoglobin (VHb) effects is intermediated via an oxygen trapping action. This may drive the enrichment of ATP production, which is mostly required for higher productivity of needed substances for that activity.
ALrawashdeh, I.N., Qaralleh, H., Al-limoun, M.O., Khleifat, K.M. (2019) Antibactrial Activity of Asteriscus graveolens Methanolic Extract: Synergistic Effect with Fungal Mediated Nanoparticles against Some Enteric Bacterial Human Pathogens. Journal of basic and applied Research in Biomedicine, 5(2): 89-98
Abstract:Antibactrial activity of Asteriscus graveolens methanolic extract and its synergy effect with fungal mediated silver nanoparticles (AgNPs) against some enteric bacterial human pathogen was conducted. Silver nanoparticles were synthesized by the fungal strain namely Tritirachium oryzae W5H as reported early. In this study, MICs of AgNPs against E. aerogenes, Salmonella sp., E. coli and C. albicans in order were 2.13, 19.15, 0.08 and 6.38 µg/mL, respectively, while the MICs of A. graveolens ethanolic extract against the same bacteria were 4, 366, <3300 and 40 µg/mL, respectively. The MIC values at concentration less than 19.15 and 40 µg/ml indicating the potent bacteriostatic effect of AgNPs and A. graveolens ethanolic extract. Increasing in IFA was reported when Nitrofurantion and Trimethoprim were combined with Etoh extract with maximum increase in IFA by 6 and 12 folds for, respectively. Also, 10 folds increasing in IFA was reported when trimethoprim was combined with AgNPs: Etoh extract. But, there were no synergistic effect between the antifungal agents (Caspofungin and Micafungin) combined with AgNPs and or A. graveolens ethanolic extract against C. albicans. The potent synergistic effect of A. graveolens ethanolic extract and/or NPs with the conventional antibiotics is novel in inhibiting antibiotics resistant bacteria. In this study, remarkable increasing in the antibacterial activity, when the most resistant antibiotics combined with A. graveolens ethanolic extract and/or NPs was reported.
Jaafreh, M., Qaralleh, H., Al-limoun, M.O. (2019). Biological value of Centaurea damascena: Minireview. . Journal of basic and applied Research in Biomedicine, 5(2): 99-106
Abstract:The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial activity. The aim of the study was to discuss antibacterial activities of essential oil composition and methanolic extract of the same plant aerial part leaves. Thirty-seven components were characterized with 86% of oxygenated terpenes. The composition was dominated by 11.45% Fokienol, 8.8% thymol, 8.21% Alpha Terpineol, 7.24% Chrysanthemumic acid, 7.13% Terpinen-4-ol and 6.59% Borneol with a high degree of polymorphism in the occurrence of these compounds as compared with the different species of centaurea.. Free radical scavenging capacity of the C. damascna methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent .
Al Tarawneh, A., Qaralleh, H., Al-limoun, M.O., Khleifat, K.M. (2019). Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake. Journal of basic and applied Research in Biomedicine, 5(2): 107-114
Abstract:The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeroginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive B. thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. The metal uptake was dependent on the type of biosorbent with different accumulation affinities toward the tested chemical form of the copper. Cells harvested at exponential growth phase showed slightly higher Cu uptake than at stationary phase, which reflect that the Cu uptake is metabolism dependent-process. The increasing order of affinity of the cupric chloride and cupric sulphate towards the three genera were almost constant. However, where cupric nitrate was used, the copper uptake behavior in Pseudomonas aeroginosa was not changed from those results with the other two chemical forms, whereas with Enterbacter aerogenes & Bacillus thurengenesis, different Cu uptake behaviors were observed. In Bacillus thuengenesis, the copper uptake rate as a function of initial Cu concentration were shown to increase with increasing the initial concentration of Cu, constantly above 320 ppm as compared with others. In Enterobacter with cupric nitrate, different bahavior was also shown in which the copper uptake decline sharply beyond the 320 ppm. These results reflect that the Cu uptake in Pseudomonas aeroginosa is with different mechanisms as compared with other tested bacteria.
Allawi, H.H., Al Tarawneh, A., Qaralleh, H., Al-limoun, M.O., Alsharafa, K.A. (2019). Using 3,4-dichloro benzoic acid as growth substrate by Brevibacterium spp: Effect of some growth conditions. Journal of basic and applied Research in Biomedicine, 5(2): 115-123
Abstract:Brevibacterium spp.that was already isolated from wastewater treatment plant of Petra City, Jordan. While 1 and 2 mM of 3,4-dichloro benzoic acid (3,4-DCBA) were used, the biodegradation rate was 14.5 and 21.25 µM/h, respectively, and the remaining was 58% for both. The remaining ratio to the quantity of 3,4-DCBA used was taken in consideration, as a result, 1 mM concentration of 3,4-DCBA substrate was applied for the rest of experiments because of the remaining amount is 0.58 mM 3,4-DCBA while 1.15 mM remained from 2mM concentration used. Thereafter, Brevibacterium spp was grown in batch cultures using M9 minimal media plus 1 mM concentrations of 3,4-DCBA as carbon and energy source. 3,4-DCBA degradation was optimally achieved at a 37 oC incubation temperature, a pH of 7.0 and an agitation rate of 150 rpm. During carbon starvation, the increased biodegradation potential of Brevibacterium spp. was accompanied by a lessening in the acclimatization period besides enhancement of the biodegradation completion of 3,4-DCBA. The adaptation to 3,4-DCBA not only increased the degradation rate but also managed to reduce the time required for complete 3,4-DCBA degradation from 80 to nearly 40 hours. In conclusion, the acclimatized period, and carbon starvation and other optimal conditions were key factors for the enhancement of the degradation ability of 3,4-DCBA by Brevibacterium spp.
Tarawneh, K., AL-Quraishi, F.M., Qarallehb, H., Al Tarawneh, A., Al-limoun, M.O., Khleifat, K.M. (2019). Biodegradation of Chlorobenzoic Acid Substitutes, Particularly, 2- Chlorobenzoic Acid by Aeromonas hydrophila. Journal of basic and applied Research in Biomedicine, 5(2): 124-135
Abstract:Bacterium Aeromonas hydrophila (A. hydrophila) was isolated from the Petra Wastewater Treatment Plant effluent in southern Jordan. It was identified by using morphological and biochemical characteristics. A. hydrophila was found to be able of using chlorobenzoate compounds as carbon and energy source. These capabilities were with different biodegradation rates (4- chlorobenzoic acid 5µM/hr, 3,4-dichlorobenzoic acid 15.5µM/hr, 2- chlorobenzoic acid 41µM/hr and 3- chlorobenzoic acid 65µM/hr). The degradation ability was monitored through the release of chloride, disappearance of the substrate and finally the growth of bacterial cells on these substrates. A. hydrophila dioxygenases physiologically induced by chlorobenzoic acid compounds, were analyzed for both ortho or meta ring-cleavage of these aromatic compounds. Only 1, 2-dioxygenase activity was detected which suggest that the cleavage is through the ortho pathway. The best results of degradation of 2-CBA compound were obtained with 3mM substrate concentration, 25ºC, pH 7and 200µl inoculum size. The carbon sources affected the 2-CBA degradation differently from that on chloride and cell mass production. Nitrogen sources used reduced the degradation activity of the 2-CBA as well as in the chlorine release from 2-CBA. However, the nitrogen source L-proline had a slight enhancement effect on the biodegradation between the 40-80h.