Volume (6), Issue (1), (2020): 1

Oladele, J.O., Adewale, O.O., Oyewole, O.I., Gbolagbade, A., Oyeleke, M.O. (2020) Assessment of the Protective Effects of Vitamin C and E on Cypermethrin-induced Nephrotoxicity and Electrolyte Imbalance in Wistar Rats. Journal of basic and applied Research in Biomedicine, 6(1): 1-6

Abstract:

Cypermethrin is a potent pyrethroids insecticide causing different pathological features when exposed to mammal. Vitamins are used as nutrient supplements and in clinical studies as medical intervention in some disease conditions. This study was designed to investigate the possible protective effect of vitamin C and E on Cypermethrin induced nephrotoxicity in wistar albino rats. Twenty-eight (28) wistar albino rats were sorted into four groups of seven rats per groups were used in this study. Group A serves as the control and received distilled water orally. Group B, C and D were administered 25mg/kg body weight cypermethrin orally. Group C and D were treated daily with 40mg/kg body weight vitamin C and 20mg/kg body weight vitamin E respectively by oral administration while group B was left untreated for 14 days. Cypermethrin significantly (P<0.05) induced nephrotoxicity as characterized with significant increased (P<0.05) in the serum levels of Urea, uric acid and creatinine. It also caused significant decrease (P<0.05) in renal total protein, albumin and globulin. Exposure to Cypermethrin induced electrolyte imbalance in rats with significant increase in serum chloride ion, potassium ion and significant decrease in serum level of sodium ion and bicarbonates. Histological results revealed that cypermethrin caused distortion in histoarchitecture of the kidney characterized by lesion of glomerulus, damaged Bowman’s capsule, degenerated and vacuolated renal tubules. Taken together, vitamin C and E significantly reverse all these alterations and offer protection to the kidney membrane.

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Alqudah, A., Ja`afreh, W. (2020) 3,4-Dichlorobenzoic acid biodegradation by the Edwardsiella tarda: Effect of Some Growth Conditions. Journal of basic and applied Research in Biomedicine, 6(1): 7-14

Abstract:

The biodegradation of 3,4-DiChlorobenzoic acid was investigated by using Edwardsiella tarda and it used 3,4-DCBA as sole carbon and energy source. Several concentrations of 3,4-D CBAs (1mM, 2mM ,3mM ,4mM and 5mM) were used. The highest rate of degradation of 3,4-D CBAs was obtained at a concentration (2mM). The experiments were included substrate concentration, temperature, pH, starvation, adaptation, carbon and nitrogen sources. The degradation ability was monitored through the release of chloride disappearance of the substrate and finally the growth of bacterial cells on that substrate. The optimal temperature and pH for the bacteria were 42ºC and 7.5, respectively. Adaptation of the cells on 3,4-DCBA for 48 hours and cells starvation for 24 hours and 48 hours increasing the initial degradation rate. The carbon sources affected the 3,4 –DCBA degradation differently from that on chloride and cell mass production. Nitrogen sources supplied (yeast extract, L-proline, casein, NH4, K-Nitrate, arginine, urea and glycine). Urea and casine caused a repression in 3,4-DCBA degradation. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 3,4-DCBA is catabolized by ortho-ring cleavage pathway.

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