Effect of Rosemary Extract and Vitamin E on Lipid Peroxidation and the Quality During Chilling and Frozen Storage of Fried Nile Tilapia Fillets (Oreochromis niloticus)

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Ibrahim Fouad
Mohamed Madi
Sabah Lamlom
Idress Attitalla


Antioxidants are added to fresh and processed meat to prevent lipid oxidation, retard development of off-flavors, and improve color stability. Synthetic antioxidants have been confirmed for their toxicological and carcinogenic effects. Thus, the food industry now chooses natural products over synthetic ones. This study was carried out to investigate the effect of Rosemary extract (R.E.) and vitamin E on the quality of fried fillets of Nile tilapia (Oreochromis niloticus) during chilling and frozen storage. Fillets of Nile tilapia were treated with (R.E.)  0.1%, 0.2%, 0.3% and Vitamin E 0.1% then stored for 5,10 and 15 days at 4±1°C and for three months at -18±2°C. Then chemical tests including Peroxide value (PV), Thio-barbituric acid (TBA), Tri-methylamine-nitrogen (TMA-N) and Total volatile base-nitrogen (TVB-N) were done to evaluate the preservative effect of (R.E.) and vitamin E during storage. The TBA and PV increased in all treatments due to lipid peroxidation. Results showed that TMA-N,TVB-N, value of (R.E.) and vitamin E treated samples were significantly lower than those of the control samples (P<0.05). Results of our investigation revealed that R.E. retarded oxidative changes in chilling and frozen fried fillets of Nile tilapia whereas R.E. 0.1%, 0.2% and vitamin E 0.1% were not as effective as R.E. 0.3%  on oxidative stability. Best oxidation inhabitation results on chilling and frozen fried fillets of Nile tilapia was obtained when employing of R.E. The obtained results also showed that there was a significant (p<0.05) enhancement in sensory quality attributes of fried fillets of Nile tilapia; samples treated with R.E. and vitamin E. Conclusion, The tested R.E. had a high effectiveness as antioxidative and antimicrobial should be utilized for extending the shelf-life through retarded the spoilage and enhancing quality attributes of fried fillets of Nile tilapia during chilling and frozen storage.


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Akhtar, P., Gray, J., Booren, A. and Garling, D. (1998) Effect of dietary components and surface application of oleoresin RE in lipid stability of rainbow trout (Oncorhynchus mykiss) muscle during refrigerated and frozen storage. Journal of Food lipids, 5: 43–45.

Al-saghir, S., Thurner, K., WangerFrisch, K. and Luf, W. (2004). Effects of different cooking procedure on lipid quality and cholesterol oxidation of farmed salmon fish (Salmo salar). Journal of Agriculture Food Chemistry, 52: 5290-5296.

Ansorena, D., Guembe, A. and Mendizábal, T. (2010) Astiasarán effect of fish and oil nature on frying process and nutritional product quality. J Food Sci., 75 (2): H62-7.

AOAC. (2005) Official methods of analysis (18th ed.), Association of Official Analytical Chemists International, Maryland, USA.

Aro, T., Tahvonen, R., Mattila, T., Nurmi, J., Sivonen, T. and Kallio, H. (2000) Effects of season and processing on oil content and fatty acids of Baltic herring (Clupea harengus membras). J. Agric. Food Chem., 48: 6085-6093.

Aro, T., Tahvonen, R., Mattila, T., Nurmi, J., Sivonen, T.and Kallio, H. (2000). Effects of season and processing on oil content and fatty acids of Baltic herring (Clupea harengus membras). J. Agric. Food Chem., 48: 6085-6093.

Aubourg, S., Perez-Alonso, F. and Gallardo, J. (2004) Studies on rancidity inhibition in frozen horse mackerel (Trachurus trachurus) by citric acid and ascorbic acids. European Journal of Lipid Science and Technology, 106: 232-240.

Aubourg, S., QuitralAngelica, V., Larra?n, M., Rodr?guez, A., Gomez, J., Maier, L. and Vinagre, J. (2007) Autolytic degradation and microbiological activity in farmed coho salmon (Oncorhynchus kisutch) during chilled storage. Food Chemistry, 104: 369–375.

Aubourg, S., Rodriguezand A., Gallardo J., (2005) Rancidity development during frozen storage of mackerel (Scomber scombrus): effect of catching season and commercial presentation. European Journal of Lipid Science and Technology, 107: 316- 323.

Aubourg, S., Rodriguezand, A. and Gallardo, J. (2005) Rancidity development during frozen storage of mackerel (Scomber scombrus): effect of catching season and commercial presentation. European Journal of Lipid Science and Technology, 107: 316- 323.

Benjakul, S., Visessanguan, W., Phongkanpai, V. and Tanaka, M. (2005) Antioxidative activity of caramelisation products and their preventive effect on lipid oxidation in fish mince. Food Chemistry, 90; 231-239.

Ciriano, MG-Id, Garc´?a-Herreros, C, Larequi, E, Valencia, I, Ansorena, D, Astiasar´an, I. (2009). Use of natural antioxidants from lyophilized water extracts of Borago officinalis in dry fermented sausages enriched in ?-3 PUFA. Meat Sci 83:271–7.

Ciriano, MG-Id, Rehecho, S, Calvo, M.I, Cavero, R.Y, Navarro, I., Astiasaran, I., Ansorena, D. (2010) Effect of lyophilized water extracts of Melissa officinalis on the stability of algae and linseed oil-in-water emulsion to be used as a functional ingredient in meat products. Meat Sci 85:373–7.

Corbo, M. R., Bevilacqua, A., Campaniello, D., Amato, D., Speranza, B. and Sinigaglia, M.(2009) Prolonging microbial shelf-life of foods through the use of natural compounds and non-thermal approaches-a review. International Journal of Food Science and Technology, 44: 223-241.

Darweash, B.M., 1996. Chemical composition of kobebah from carp and bolti fish. Egypt. J. Food Sci., 24 (3): 345-358.

Dimitrios, G. , Georgios, B., Panagiota, K., Ioannis, A., and Dimitrios, J. (2007):Effect of rosemary extract, chitosan and ?-tocopherol on lipid oxidation and colour stability during frozen storage of beef burgers. Meat Science ,Volume 75, Pages 256-264

Duncan, D.B. (1955) Multiple range and F test. Biometrics, 11: 1-42.

Elagba Mohamed, H.A., Al-Maqbaly, R. and Mansour H. (2010) Proximate composition, amino acid and mineral contents of five commercial Nile fishes in Sudan. African Journal of Food Science, 4(10): 650-654.

Elsonbaty, S. M., Eltahawy, N., Al-dmour, S. M., Easa, S., & Qaralleh, H. (2020). Ionizing Radiation effect on Teucrium polium: Phytochemical Contents antioxidant and antibacterial Activity. Arab Journal of Nuclear Sciences and Applications, 53(2), 98-110.

Erkan, N., Ozden, O. and Selçuk, A. (2010) Effect of frying, grilling, and steaming on amino acid composition of marine fishes. J. Med. Food, 13 (6): 1524-31.

Erkan, N., Tosun, S.Y., Ulusoy, S. and Üretener, G. (2011). The use of thyme and laurel essential oil treatments to extend the shelf life of bluefish (Pomatomuss altatrix) during storage in ice. J. Verbr. Lebensm, 6: 39-48.

Fagan, J.D., Gormley, R.T. and Huircheartaigh, M. (2003) Effect of freeze-chilling, in comparison with fresh, chilling and freezing, on some quality parameters of raw whiting, mackerel and salmon portion. Food Science and Technology, 36: 647-655.

Feldberg, R., Chang, S., Kotik, A., Nadler, M., Neuwirth, Z., Sundstorm, D. and Thompson, N. (1988) In vitro mechanism of inhibition of bacterial cell growth by allicin. Antimicrob Agents Ch., 32(12): 1763–1768.

Goudlas, A.E. and Kontaminas, M.G.(2005) Effect of salting and smoking-method on the keeping quality of chub mackerel (Scomber japonicus): biochemical and sensory attributes. Food Chemistry, 93: 511-520.

Iebrahim, S.M. and EL-Sherif, S. A. (2008) Effect of some plant extracts on quality aspects of frozen Tilapia (Oreochromis niloticus L.) fillets. Global Veterinaria, 22: 62-66.

Inatani, R., Nakatani, H., Fuwa, H. and Seto, H. (1983) The stereochemistry of the OH group on C-7 rosmanol has been revealed to be a configuration as the structure 1 on the basis of a re-examination of the NOE analysis. Journal of Agriculture Bio Chemistry, 47: 521–528.

Jaafreh, M., Khleifat, K. M., Qaralleh, H., & Al-limoun, M. O. (2019). Antibacterial and Antioxidant Activities of Centeurea damascena Methanolic Extract. Journal of Basic and Applied Research in Biomedicine 5(1): 55-63

Kaya, A., Tekin, A. R., & Öner, M. D. (1993). Oxidative stability of sunflower and olive oils: comparison between a modified active oxygen method and long term storage. LWT-Food Science and Technology, 26(5), 464-468.

Khan, M. A., Parrish, C. C., & Shahidi, F. (2006). Effects of mechanical handling, storage on ice and ascorbic acid treatment on lipid oxidation in cultured Newfoundland blue mussel (Mytilus edulis). Food chemistry, 99(3), 605-614.

Kirk, S., & Sawyer, R. (1991). Pearson's composition and analysis of foods (No. Ed. 9). Longman Group Ltd..

Leung, K. S., Leung, H. H., Wu, C. Y., Galano, J. M., Durand, T., & Lee, J. C. Y. (2019). Limited antioxidant effect of rosemary in lipid oxidation of pan-fried salmon. Biomolecules, 9(8), 313.

Leung, K. S., Leung, H. H., Wu, C. Y., Galano, J. M., Durand, T., & Lee, J. C. Y. (2019). Limited antioxidant effect of rosemary in lipid oxidation of pan-fried salmon. Biomolecules, 9(8), 313.

Maqsood, S., & Benjakul, S. (2011). Comparative studies on molecular changes and pro-oxidative activity of haemoglobin from different fish species as influenced by pH. Food Chemistry, 124(3), 875-883.

Masuda, T., Inaba, Y., & Takeda, Y. (2001). Antioxidant mechanism of carnosic acid: structural identification of two oxidation products. Journal of Agricultural and Food Chemistry, 49(11), 5560-5565.

Mei, J., Ma, X., & Xie, J. (2019). Review on natural preservatives for extending fish shelf life. Foods, 8(10), 490.

Musaiger, A. O., & D'Souza, R. (2008). The effects of different methods of cooking on proximate, mineral and heavy metal composition of fish and shrimps consumed in the Arabian Gulf. Archivos latinoamericanos de nutrición, 58(1).

Nakatani, N. (1994). Antioxidative and antimicrobial constituents of herbs and spices. Spices, herbs and edible fungi.

Nieto, G., Ros, G. and Castillo, J. )2018(:Antioxidant and Antimicrobial Properties of Rosemary (Rosmarinus officinalis, L.) Medicines journal, 5, 98; (1-13).

Nikoo, M., Ghomi, M. R., Rahimabadi, E. Z., Benjakul, S., & Javadian, B. (2010). The effects of deep-frying, refrigerated storage and reheating on the fat content, oxidation and fatty acid composition of the fish Rutilus frisii kutum. Journal of Food Processing and Technology, 1(1): 1-4

Oladele, J., Adewale, O., Oyewole, O., Gbolagbade, A., & Oyeleke, M. (2020). Assessment of the Protective Effects of Vitamin C and E on Cypermethrin-induced Nephrotoxicity and Electrolyte Imbalance in Wistar Rats. Journal of Basic and Applied Research in Biomedicine, 6(1), 1-6.

Ramadan, K., & Alshamrani, S. (2016). Phytochemical analysis and antioxidant activity of Salvadora persica extracts. Journal of Basic and Applied Research in Biomedicine, 2(3), 390-395.

Pazos, M., Gallardo, J. M., Torres, J. L., & Medina, I. (2005). Activity of grape polyphenols as inhibitors of the oxidation of fish lipids and frozen fish muscle. Food Chemistry, 92(3), 547-557.

Puwastien, P., Judprasong, K., Kettwan, E., Vasanachitt, K., Nakngamanong, Y., & Bhattacharjee, L. (1999). Proximate composition of raw and cooked Thai freshwater and marine fish. Journal of Food Composition and Analysis, 12(1), 9-16.

Raju, C. V., Naik, A. T. R., & Ramesha, T. J. (1998). Development of ready-to-fry fish cutlet from pink perch Nemipterus japonicus. Indian J Nutr Diet, 36, 23-28.

Rižnar, K., ?elan, Š., Knez, Ž., ŠKerget, M., Bauman, D., & Glaser, R. (2006). Antioxidant and antimicrobial activity of rosemary extract in chicken frankfurters. Journal of Food Science, 71(7), C425-C429.

Rodríguez, A., Carriles, N., Cruz, J. M., & Aubourg, S. P. (2008). Changes in the flesh of cooked farmed salmon (Oncorhynchus kisutch) with previous storage in slurry ice (? 1.5 C). LWT-Food Science and Technology, 41(9), 1726-1732.

Rostamzad, H., Shabanpour, B., Shabani, A., & Shahiri, H. (2011). Enhancement of the storage quality of frozen Persian sturgeon fillets by using of ascorbic acid. International food research journal, 18(1): 109-116.

Sahari, M. A., Nazemroaya, S., & Rezaei, M. (2009). Fatty acid and biochemical changes in mackerel (Scomberomorus commerson) and shark (Carcharhinus dussumieri) fillets during frozen storage. American-Eurasian Journal of Sustainable Agriculture, 3(3), 519-527.

Santerre, C. R., Ingram, R., Xu, D. H., Lewis, G. W., & Lane, L. G. (2000). Chlordane and toxaphene residues following cooking of treated channel catfish fillets. Journal of food protection, 63(6), 763-767.

Sarabi, M., Keramat, J., & Kadivar, M. (2017). Antioxidant effect of rosemary extract and BHT on the quality of coated fried Escolar (Lipidocybium flavobrunium) fish fillets during frozen storage. International Food Research Journal, 24(2), 525.

SAS Institute. (2000). JMP: Statistics and graphics guide. Sas Inst.

Schubring, R. (1996). Colour measurement on battered and breaded fish products. Informationen fuer die Fischwirtschaft (Germany) 43 (2): 84-88.

Schwarz, K., & Ternes, W. (1992). Antioxidative constituents of Rosmarinus officinalis and Salvia officinalis. I. Determination of phenolic diterpenes with antioxidative activity amongst tocochromanols using HPLC. Zeitschrift fur Lebensmittel-untersuchung und-forschung, 195(2), 95-98.

Selmi, S. A. L. A. H., & Sadok, S. A. L. O. U. A. (2008). The effect of natural antioxidant (Thymus vulgaris Linnaeus) on flesh quality of tuna (Thunnus thynnus (Linnaeus)) during chilled storage. Pan-American Journal of Aquatic Sciences, 3(1), 36-45.

Serdaro?lu, M., & Feleko?lu, E. (2005). Effects of using rosemary extract and onion juice on oxidative stability of sardine (Sardina pilchardus) mince. Journal of Food Quality, 28(2), 109-120.

Shelef, L. A., Naglik, O. A., & Bogen, D. W. (1980). Sensitivity of some common food?borne bacteria to the spices sage, rosemary, and allspice. Journal of Food Science, 45(4), 1042-1044.

Singh, G., Marimuthu, P., Murali, H. S., & Bawa, A. S. (2005). Antioxidative and antibacterial potentials of essential oils and extracts isolated from various spice materials. Journal of food safety, 25(2), 130-145.

Stevenson, S. G., Vaisey-Genser, M., & Eskin, N. A. M. (1984). Quality control in the use of deep frying oils. Journal of the American Oil Chemists Society, 61(6), 1102-1108.

Taheri, S., Motallebi, A. A., Fazlara, A., Aftabsavar, Y., & Aubourg, S. P. (2012). Effect of previous ascorbic acid treatment on the fatty acid profile of cobia (Rachycentron canadum) fillets during frozen storage. Grasas y Aceites, 63: 70-78.

Teeny, F. M., & Miyauchi, D. (1972). Preparation and utilization of frozen blocks of minced black rockfish muscle. Journal of Milk and Food Technology, 35(7), 414-417.

Verma, J. K., & Srikar, L. N. (1994). Protein and lipid changes in pink perch (nemipterus-japonicus) mince during frozen storage. Journal of Food Science and Technology, 31: 238-240.

Vidya, S. R. G., & Srikar, L. N. (1996). Effect of preprocess ice storage on the lipid changes of Japanese threadfin bream (Nemipterus japonicus) mince during frozen. Asian Fisher Sci, 9, 109-114.

Wu, W. H., & Lillard, D. A. (1998). Cholesterol and proximate composition of channel catfish (ictalurus punctatus) fillets–changes following cooking by microwave heating, deep?fat frying, and oven baking. Journal of food quality, 21(1), 41-51.

Yasin, N. M., & Abou-Taleb, M. (2007). Antioxidant and antimicrobial effects of marjoram and thyme in coated refrigerated semi fried mullet fish fillets. World Journal of Dairy & Food Sciences, 2(1), 1-9.

Zakipour Rahimabadi, E., & Divband, M. (2012). The Effects of Coating and Zataria multiflora Boiss Essential Oil on Chemical Attributes of Silver Carp Fillet Stored at 4ºC. International Food Research Journal, 19(2).

Zarei, M., Fazlara, A., Jamaledin, D., & Mojtahedi, M. (2011). Microbial and chemical quality of vacuumpacked frozen fillets of narrow-barred Spanish mackerel (Scomberomorus commerson) and silver pomfret (Pampus argenteus) marketed in Iran. World Journal of Fish and Marine Science, 3, 410-413.