Ameliorative effect of fermented Pentaclethra macrophylla (African oil bean seed) on high fat diet and sucrose drink induced metabolic syndrome in male New Zealand rabbits.

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Nwozo Onyenibe
Daramola Victoria
Nwawuba Udogadi

Abstract

The consumption of nutraceuticals has become progressively popular in improving health, as well as disease treatment and prevention. The aim of this study is to evaluate the ameliorative effect of fermented Pentaclethra macrophylla (African oil bean) seed on high fat diet and sucrose drink induced metabolic syndrome in male New Zealand rabbits. All experimental procedures were carried using standard methods. A total of twenty (20) rabbits were used for this study, the animals were randomly grouped into five (5) groups with four (4) animals each. Metabolic syndrome risk factors obesity, hypertension, dyslipidemia and hyperglycemia were determined. HFD+30%SUC had over 1500g body weight (bwt) and fasting blood sugar (FBS) 239.50±5.74 mg/dl  relative to the normal control with 1000±6.73g and 77.00±5.94 respectively whereas ND+30%SUC had lower FBS (l172.50±2.52) compared to HFD+30%SUC. There was a significant p<0.05 increase in body weights in treated groups with 1274±11.19g, 1282.75±4.99g, 1285±4.76g and FBS levels in mg/dL198.25±4.79, 198.75±8.06, 200±3.74 in rabbits  compared to untreated HFD+30%SUC. Glycated hemoglobin, and ?-Amylase were significantly p<0.05 elevated, glucose-6-phosphate dehydrogenase levels were significantly p<0.05 reduced, lipid profile markers (HDL, TRIG & CHOL), electrolytes (sodium, potassium, calcium), urea and creatinine levels were significantly p<0.05 altered in HFD+30%SUC exposed group relative to the normal control and ND+30%SUC. However, dietary supplementation with fermented Pentaclethra macrophylla seeds caused weight, FBS, serum lipid reduction and potentiated electrolyte, urea and creatinine levels. Therefore, according to our results, fermented Pentaclethra macrophylla seed is an excellent nutraceutical and its consumption should be encouraged in modifying high fat and elevated intake of sucrose in rabbit

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Lee, S.E., Han, K., Kang, Y.M., Kim, S.O., and Cho, Y.K., et al. (2018). Trends in the prevalence of metabolic syndrome and its components in South Korea: Findings from the Korean National Health Insurance Service Database (2009-2013). PLoS ONE. 13(3): 1-12.

Wong, S.K., Chin, K.Y., Farihah, H.S., Ahmad, F., and Soelaiman, I.N. (2016). Animal models of metabolic syndrome: a review. Nutrition & Metabolism. 13(65): 1-12.

Ranasinghe, P., Cooray, D.N., Jayawardena, R., and Katulanda, P. (2015). The influence of family history of hypertension on disease prevalence and associated metabolic risk factors among Sri Lankan adults. BMC Public Health. 15:576.

Wagner, A., Dallongeville, J., Haas, B., Ruidavets, J.B., Amouyel, P., et al. (2012). Sedentary behaviour, physical activity and dietary patterns are independently associated with the metabolic syndrome. Diabetes Metab. 38:428–35.

Alberti, K.G., Zimmet, P., and Shaw, J. (2006). Metabolic syndrome-a new world-wide definition: A Consensus Statement from the International Diabetes Federation. Diabet Med. 23(5): 469-80.

Gancheva, S., Zhelyazkova-Savova, M., Galunska, B., Chervenkov, T. (2015). Experimental Models of Metabolic Syndrome in Rats. Scripta Scientifica Medica. 47(2): 23-30.

Anaka, O.N., Owolabi, O.J., and Emenike, C.F. (2013). Anti-hyperlipidemic effect of aqueous leaf extract of Emilia praetermissa milne-redh (Asteraceae) in rats. International Journal of Biosciences (IJB). 3(5): 68-77.

Lebeau, N.O., Amang, A.P., Mezui, C., Nkwengoua, Z.E., Enow-Orock, E.G., et al. (2017). Toxicological Studies of Ethanolic Extract of Emilia praetermissa Milne-Redh (Asteraceae) in Rats. Journal of Advances in Biology & Biotechnology. 11(4): 1-14.

Nwozo, S.O., Adeneye, D.A., and Nwawuba, S.U. (2018). Effect of Solanium melongena fruits supplemented diet on hyperglycemia, overweight, liver function and dyslipidemia in male New Zealand rabbits fed high fat and sucrose diet. Integrative Obesity and Diabetes. 4(3): 1-5.

Nwawuba, S.U., and Okechukwu, F.C. (2018). The effect of Cyperus esculentus (Tigernut) oil on liver, kidney and hematological biomarkers in low dose streptozocin and high fat diet exposed male wistar rats. International Journal of Food Science and Nutrition. 3(4): 148-152.

Woo, K., Chook, P., Chan, L., Cheung, A., and Fung, W.H. (2006). Long-term improvement in homocysteine levels and arterial endothelial function after 1-year folic acid supplementation. American Journal of Medicine. 7:535-539.

Anioke, I.C. (2019). Effect of Fermented Pentaclethra Macrophylla Benth (African Oil Bean) Seed Extract on Plasma Lipid Profile in Healthy Rat Model-A Preliminary Study. South Asian Research Journal of Natural Products. 2(1): 1-9.

Igwenyi, I.O., Isiguzo, O.E., Aja, P.M., Ugwu Okechukwu, P.C., Ezeani N.N., and Uraku, A.J. (2015). Proximate Composition, Mineral Content and Phytochemical Analysis of the African Oil Bean (Pentaclethra macrophylla) Seed. American-Eurasian J. Agric. & Environ. Sci. 15(9): 1873-1875.

Nwanjo, H., Iroagba, I., Nnatuanya, I., and Eze, N. (2006). Is fermented pentactethra macrophylla nutritional or antinutritional: Response from haematological studies in protein malnourished guinea Pigs. The Internet Journal of Nutrition and Wellness. 4:(2).

Okwuonu, G.C., Ojimadu, N.E., Ozoemelam, K., Esione, A., Iwe, I.S., et al. (2013). Case Report the Potential Anti Hypertensive effects of the African Oil-bean Seed - Pentaclethra Macrophylla Bentham. Pioneer Medical Journal. 3(5): 1-11.

Okwu, D.E., and Aluwuo, C.J. (2008). Studies on the Phytochemical Composition and Fermentation of the Seeds of African Oil Bean Tree (Pentaclethra Macrophylla Benth). Int. J. Chem. Sci. 6(2): 773-788.

Harbone, J.B. (1998). Phytochemical methods-A guide to modern techniques of plants analysis. Champman and Hall, London. Pp. 182-190.

Yin, W., Yuan, Z., Wang, Z., Yang, B., and Yang Y. (2002). A diet high in saturated fat and sucrose alters glucoregulation and induces aortic fatty streaks in New Zealand white rabbits. Int. J. Exp. Diabetes Res. 3(3):179–184.

Loscalzo, J., Fauci, A.S., Braunwald, E., Dennis, L., Kasper, H., et al. (2008). Harrison's Principles of Internal Medicine. McGraw-Hill Medical Pubs.

Olaiya, C.O., Choudhary, M.I., Ogunyemi, O.M.., and Nwauzoma, A.B. (2013). Nutraceuticals from Bitter Leaf (Vernonia amygdalina Del.) Protects against Cadmium Chloride induced Hypertension in Albino Rats. Nature and Science. 11(6): 136-145.

Yang, Z.H., Miyahara, H., Takeo, J., and Katayama, M. (2012). Diet high in fat and sucrose induces rapid onset of obesity-related metabolic syndrome partly through rapid response of genes involved in lipogenesis, insulin signalling and inflammation in mice. Diabetol Metab Syndr. 4:32.

Li, Y., Lu, Z., Zhang, X., Yu, H., Kirkwood, K.L. et al. (2015). Metabolic syndrome exacerbates inflammation and bone loss in periodontitis. J Dent Res. 94:362–70.

Aguilera, A.A., Diaz, G.H., Barcelata, M.L., Guerrero, O.A., and Ros, R.M. (2004). Effects of fish oil on hypertension, plasma lipids, and tumor necrosis factor-alpha in rats with sucrose-induced metabolic syndrome. J Nutr Biochem. 15:350–7.

Ufelle, S.A., Neboh, E.E., Achukwu, P.U., and Ghasi, S.S. (2015). Potential haematopoietic properties of crude methanolic seed extract of Pentaclethra macrophylla in Wistar rats. (Biokemistri) An International Journal of the Nigerian Society for Experimental Biology. 27(1): 22–25.

Nwozo, S.O., and Nwawuba, S.U. (2019). Ameliorative Potentials of Cyperus Es¬culentus Oil on Type 2 Diabetes Induced by High Fat Diet and Low Dose Streptozotocin in Male Wistar Rats. International Journal of Diabetes Research. 2(1): 33-38.

Antonio, P., Emma, L.C., Ciriaco, C., and Giuliana, S. (2013). Glucose-6-Phos¬phate Dehydrogenase Deficiency and Diabetes Mellitus with Severe Retinal Complications in a Sardinian Population, Italy. Int J Med Sci. 10(13): 1907-1913.

Tundis, R., Loizzo, M.R., and Menichini, F. (2010). Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem. 10(4): 315-31.

Nwozo, S.O., Nyam, A.N., Nwawuba, S.U., Olukotun, O.I. (2019). Hypoglycemic and Antioxidant Capacity of Curcuma Longa and Viscum Album in Alloxan Induced Diabetic Male Wistar Rats. International Journal of Diabetes and Endocrinology. 4(1): 26-34.

Agada, P.O., and Braide, V.B. (2009). Effect of Dietary Garcinia Kola Seed on selected serum electrolytes and Trace Metals in male Albino rats. Nig J Physiol Sci. 24: 53-57.