Comparative Studies on the Phytochemical Constituents and in vitro Antioxidant Activities of Methanol and Ethyl Acetate Leaf Extracts of Talium Trangulare

Main Article Content

Oyedotun Oyeleke
Mutiat Bamigboye
Boyede Olowookere
Kehinde Alabi
Samuel Oladele
Johnson Oladele

Abstract

Medicinal herbs have been known for their use as an alternative medicine in the management and treatment of different categories of diseases. The present study was designed to assess the phytochemical constituents present in methanol and ethylacetate leaves extract of Talium trangulare and its antioxidant potentials against free radicals comparatively. The functional groups of the phytochemicals were carried out using FTIR techniques while the phytochemical component of the extract was determined by standard methods. The comparative studies of antioxidant abilities of methanol and ethylacetate leaves extracts of Talium trangulare were evaluated by various antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide, hydroxyl radicals, scavenging activities. These different antioxidant activities were compared with standard antioxidants compounds such as ascorbic acid and Gallic acid. The total phenolic content in ethylacetate extract is higher than what was obtained from methanol extract of Talium trangulare, however, methanol extract has higher tannins and flavonoids content. The methanol extract was found to have demonstrated high reduction capability and powerful free radical scavenging, especially against DPPH radical and hydrogen peroxide as compared with the ethylacetate leaves extract. The results that was obtained in the present study clearly established the antioxidant potency of both ethylacetate and methanol leaf extracts of Talium trangulare. The methanol extract shows better antioxidant activities and more flavonoids and tannin than the ethylacetate extract. In conclusion, the methanol leaf extract of Talium trangulare has comparatively better in vitro antioxidant potentials with corresponding phytochemical content than ethylacetate extract.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Article Details

Section
Articles

References

Ajanal, M, Gundkalle M and Nayak S. (2012). Estimation of total alkaloid in chitrakadivati by UV-spectrophotometer. Ancient Science of life. 31(4): 198-201

Alabri, T.H.A., A.H.S. Al Musalami, M.A. Hossain, A.M. Weli and Q. Al-Riyami, (2014). Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel. L. J. King Saud Univ. Sci. 26: 237-243.

Chao P-Y, S.-Y. Lin, K.-H. Lin et al., (2014). “Antioxidant activity in extracts of 27 indigenous Taiwanese vegetables,” Nutrients. 6 (5): 2115–2130,

Courtneya, R. J. Sirdaartaa,b, B. Matthewsc, I. E. Cock. (2015). Tannin components and inhibitory activity of Kakadu plum leaf extracts against microbial triggers of autoimmune inflammatory diseases. Pharmacognosy Journal. 7(1): 18-31.

Das, K, Tiwari, RKS, Srivastava DK. (2010). Techniques for evaluation of medicinal plant products as antimicrobial agent. Current method and future trends. Journal of medicinal plants Res. 41: 104-111.

Hannah Hepsibah, G. Jeya Jothi. (2016). A comparative study in the effects of solvents in the phytochemical profile and biological potential of ormocarpum cochinchinese auct non (LOUR). International Journal of Pharmacy and Pharmalogical science. 9(1): 67-72.

Hatano, T., Edamatsu, R., Hiramatsu, M., Mori, A., Fujita, Y., 1989. Effects of tannins and related polyphenols on superoxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37: 2016–2021.

Ilhami G. I., Haci A. A. and Mehmet C. (2005). Determination of in vitro antioxidant and radical scavenging activities of Propofol. Chem Pharmacol Bull. 53(3): 281-285.

Iloki-Assanga SB, Lewis-Luján LM, Lara-Espinoza CL, Gil-Salido AA, Fernandez-Angulo D, Rubio-Pino JL, Haines DD. (2015). Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Research Notes. 8: 1–14.

Ju, S.P., Liao, M.L., Yang, S.H. and Lee, W.J. (2007). Hydrogen-bond dynamics of interior and surface molecules in a water nanocluster: Temperature and size effects. Molecular Physics. 105(4): 429–438.

Kar, A. (2007). Pharmacognosy and Pharmacobiotechnology (Revised-Expanded Second Edition). New Age International Limted Publishers New Delhi. pp 332-600

Kedare, S.B. and Singh, R.P. (2011). Genesis and development of DPPH method of antioxidant assay. J. Food Sc. Technol. 48(4): 412-422.

Klein S.M, Cohen G., Cederbaum A.I. (1981). Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochem, 20: 6006–6012.

Kumaran, A. and Karunakaran, R.J., (2007). In vitro antioxidant activities of methanolic extract of Phyllanthus species from India. LWT-Food Science and Technology, 40: 322-352.

Mahdi-Pour, B., Jothy, S.L, Latha, L., Chen, Y and Sasidharan S. (2012). Antioxidant activity of methanol extracts of different parts of Lantana cannar. Asian pacific journal of tropical biomedium. 2(12): 960-965

Mensor, L. L., Menezes, F. S., Leitão, G. G., Reis, A. S., Santos, T. C. D., Coube, C. S., & Leitão, S. G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research. 15 (2): 127-130.

Metrouh-Amir H, Duarte CMM, Maiza F. (2015). Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial Crops and Product. 67: 249–56.

Nagendran Balasundram, Kalyana Sundram, Samir Samman. (2006). Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem. 99: 191–203.

Naima, R., Oumam, M., Hannache, H., Sesbou, A., Charrier, B., Pizzi, A., & Charrier-El Bouhtoury, F. (2015). Comparison of the impact of different extraction methods on polyphenols yields and tannins extracted from Moroccan Acacia mollissima barks. Industrial Crops and Products. 70: 245-252.

Oladele JO, Ajayi EIO, Oyeleke OM, Oladele OT, Olowookere BD, Adeniyi BM, Oyewole OI, Oladiji AT. (2020)a. A systematic review on COVID-19 pandemic with special emphasis on Curative potentials of medicinal plants. Heliyon. 6: 1-17. https://doi.org/10.1016/j.heliyon.2020.e04897

Oladele JO, Bamigboye MO, Olowookere BD, Oyeleke OM, Anyim JC, Oladele KS, Oyewole IO. (2020)b. Identification of bioactive chemical constituents present in the aqueous extract of Telfairia occidentalis and its in vitro antioxidant activities. Journal of Natural & Ayurvedic Medicine. 4(2): 1-10.

Oladele JO, Oyeleke OM, Oladele OT, Oladiji AT. (2021). Covid-19 treatment: Investigation on the phytochemical constituents of Vernonia amygdalina as potential Coronavirus-2 inhibitors. Computational toxicology. 18. 100161. https://doi.org/10.1016/j.comtox.2021.100161

Oladele, JO., Adewale, OO., Oyeleke, OM., Oyewole IO., Salami, MO., Owoade G. (2020)c. Annona muricata protects against cadmium mediated oxidative damage in brain and liver of rats. Acta facultatis medicae Naissensis. 37 (3): 252-260. DOI:10.5937/afmnai2003252O.

Oyaizu M. (1986). Studies on product of browning reaction prepared from glucose amine. Japan J Nutr. 44: 307-315.

Sasidharan, S, Chen Y, Saravanan D, Sundaram KM, Yoba, Lathal. (2011). Extraction, isolation and characterization of bioactive compounds from plants extraction. African journal of traditional altern medicine; 8:1-10

Shi, Y., Lan, Y., Matson, C., Mulligan, P., Whetstine, J.R., Cole, P.A., Casero, R.A., Shi, Y., (2004). Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 119 (7): 941–953.

Sripad, G., Prakash, V., & Rao, M. S. N. (1982). Extrability of polyphenols of sunflower seed in various solvents. Journal of Biosciences, 4(2): 145-152.

Zhao, Y.S. Chen, Y. Wang, C. L, J. Wang, and J. Lu. (2018). “Effect of drying processes on prenylflavonoid content and antioxidant activity of Epimedium koreanum Nakai.” Journal of Food and Drug Analysis. 26(2): 796–806.

Z?otek, U, S. Mikulska, M. Nagajek, and M. Swieca. (2016). “Effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) Extracts. Saudi Journal of Biological Sciences. 23(5): 628–633.