Formulation and evaluation of antimicrobial gel of cow urine distillate

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S. Shewale
S. Majumdar
N. Aloorkar
S. Rathi


In India, medicines based on herbal origin have been the basis of treatment and cure for various diseases. The Indian Cow, Bos indicus, is a most veneered and valuable animal in religious scriptures. Cow urine has found therapeutic applications since days of yore. Cow urine based preparations have powerful antimicrobial, antiviral, anti allergic, and antioxidant activity. So the current research is mainly centered on the exploration of the antimicrobial powers of cow urine and incorporation of cow urine into gel formulation. The gels were prepared by using carbopol 940, propylene glycol, glycerin, triethanolamine, distilled water along with cow urine distillate and Azadirachta indica extract. The prepared gels were evaluated for physical appearance, spreadability, pH, Homogeneity and viscosity. The present study analyzes the antibacterial and antifungal activity of Cow Urine Distillate against the clinical pathogenic microorganisms such as Bacillus subtilis and Staphalococcus aureus. Maximum antibacterial activity was observed in Bacillus subtilis than Staphalococcus aureus. Antifungal activity of cow urine distillate was analysed against Aspergillus niger. The result revealed optimum characteristics features of gel formulation and confirm antimicrobial potential of prepared gel.


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Journal of Basic And Applied Research


Ahmad I., Mehmood Z. & Mohammad F. (1998). Screening of some Indian medicinal plants for their antimicrobial properties, J. Ethnopharmacol.62: 183-193.

Debnath S.K., Sarkar S., Janakiraman , Chakraborty S.(2009).Formulation and Evaluation of Aceclofenac Gel, International Journal of Chem Tech Research,1(2),204-207.

Kekuda T.R., Kavya R., Shrungashree R.M. & Suchitra S.V. (2007). Antifungal activity of cow urine distillate.(

Majali, I., Qaralleh, H., Idid, S., Saad, S., Susanti, D., Taher, M. & Althunibat, O. (2015a). Potential antimicrobial activity of marine sponge Neopetrosia exigua. Journal of basic and applied research 1(1), 1-13

Majali, I., Althunibat, O. and Qaralleh, H. (2015b) Antimicrobial and Immunomodulatory activities of Moringa peregrine- MINIREVIEW. Journal of Basic and Applied Research 1 (2): 55-61

Minocheherhomji F.P., Vyas B.M. (2014). Study of the antimicrobial activity of cow urine and Medicinal plant extracts on pathogenic human Microbial strains, Int. J of Adv in Pharmacy, Biology and Chemistry. 4(3), 836-840.

Misal G., Dixit G.,Gulkari V.(2012).Formulation and evaluation of herbal gel, Indian journal of natural products and resources.3(4),501-505.

Rajapandiyan K., Shanthi S., Murugan A.M. , Alagu Muthu G., Singh R. (2011). Azadirachta indica - cow urine extract, a novel controlling agent towards clinically significant Multi Drug Resistant Pathogens, Journal of Applied Pharmaceutical Science, 1 (10), 107-113.

Sarkar B., Shukla R., Devgan M. et al. (2014). Synthesis Formulation and evaluation of Antimicrobial gel of novel napthofuran derivative, Asian J. Pharm. Res. 4(3), 118-121.

Sathasivam A., Muthuselvam M., et al. (2010). Antimicrobial Activities of Cow Urine Distillate against Some Clinical Pathogens, Global Journal of Pharmacology, 4(1): 41-44.

Sai Kishore V., Lakshmana R.., Ramesh B., Aditya K. (2015). Indian Cow Urine Distillation and Therapeutic Uses, Mintage Journal of Pharmaceutical & Medical Sciences.4 (1), 1-5.

Shah, E., (1997). Herbal composition in cow urine. US Patent 5693327. Dec 2.