Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Neonates with Jaundice in a Tertiary Hospitalin Nigeria

Main Article Content

Omisakin C.T
Esan A.J
Adesoji O.A
Ogunbusuyi B.O


G6PD deficiency is known to be associated with neonatal jaundice, kernicterus and even death. G6PD is the first enzyme of the pentose phosphate pathway and catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolactone, with the concomitant reduction of nicotinamide adenine dinucleotide phosphate (NADP) to its reduced form (NADPH). Three hundred and twenty five neonatal blood samples were collected for the study between the ages of 1–10days of both sexes. About 4ml of blood sample was collected from newborn baby with jaundice, 2ml of blood sample was dispensed into di-potassium ethylenediaminetetracetic acid (K2EDTA) bottles for packed cell volume and haemoglobin estimation using haematology analyzer (sysmex model KX-21N) also K2EDTA blood sample was used for G6PD status determination, remaining 2ml of blood sample was dispensed into heparin bottles for bilirubin estimation. Out of 325 newborn babies with neonatal jaundice, 96(29.5%) were G6PD deficient; 57 were male and 39 were female. Mean± SD of total bilirubin (B1) andconjugated bilirubin(B2) were significantly (P<0.05) higher in G6PD- deficient participants compared with G6PD normal. Neonates should be screened for G6PD deficiency when family history, ethnic or geographic origin on the timing of the appearance of neonatal jaundice suggests the possibility of G6PD deficiency.


Download data is not yet available.


Metrics Loading ...

Article Details

Journal of Basic And Applied Research


Abbas Al-Omran, Fouad Al-Ghazal, Samir Gupta and Thomas B. John. Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Jaundice in Al-Hofuf Area 156 Annals of Saudi Medicine, Vol 19, No 2, 1999

Ainoon O, Alawiyah A, Yu YH, Cheong SK, Hamidah NH, Boo NY, et al. Semiquantitative screening test for G6PD deficiency detects severe deficiency but misses a substantial proportion of partially-deficient females. Southeast Asian J Trop Med Public Health. 2003;34:405–14.

Beutler E, Mitchel M. Special Modification of the Fluorescent Screening method for Glucose-6-phosphate dehydrogenase deficiency Blood (1968) pp. 32: 816.

Cappellini MD, Fiorelli G. Glucose-6-phosphate dehydrogenase deficiency. Lancet 2008;371:64–74

Cheesbrough M. Blood Transfusion Practice: Blood donation and Storage of blood, District Laboratory Practice in tropical countries, low-price edition by Cambridge Universal Press (2002) pp. 352-353.

Chinevere TD, Murray CK, Grant E Jr, Johnson GA, Duelm F, Hospenthal DR. Prevalence of Glucose-6-Phosphate Dehydrogenase Deficiency in U.S. Army Personnel. Mil Med 2006;171:905-7.

Frank JE. Diagnosis and management of G6PD deficiency. Am Fam Physician 2005;72:1277–1282

Gregg XT, Prchal JT. Red cell enzymopathies. In: Hoffman R, ed. Hematology: basic principles and practice. 4th ed. Philadelphia: Churchill Livingstone, 2000:657–60.

Hermiston ML, Mentzer WC. A practical approach to the evaluation of the anemic child. PediatrClin North Am. 2002;49:877–91.

Jendrassik L, Grof P. Modified Jendrasik-Grof method for bilirubin, Adapted to the Abbott biochromatic analyzer. Clinical chemistry by Am. Assoc. Clin. chem.(1978). 24: 1841-1845.

Kaplan M, Renbaum P, Levy-Lahad E, et al. Gilbert syndrome and glucose-6-phosphate dehydrogenase deficiency: a dose-dependent genetic interaction crucial to neonatal hyperbilirubinemia.ProcNatlAcadSci U S A 1997;94:12128–32

Kaplan M, Hammerman C. Severe neonatal hyperbilirubinemia. A potential complication of glucose-6-phosphate dehydrogenase deficiency.ClinPerinatol 1998;25:575–90

Lukens JN, GladerBE. Hereditary hemolytic anaemias associated with abnormalities of erythrocyte glycolysis and nucleotide metabolism:wintrobe’s clinical hematology 10th ed: (1999)pp. 1160-1175.

Mohanty D, Mukerjee NB, Colah RB. Glucose-6-phosphate deficiency in India. Indian J Pediatr 2004;71:525-9.

Parthasarathy A, Ramachandran P, Thangavelu S. Essential: newborn care: Selected Topics in Paediatrics for Practioners. 1 sted. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2004. p. 23-4.

RaminIranpour, MahinHashemipor, Seyed-MojtabaTalaei, Mohsen Soroshinia, AbasgholiAmini. Newborn Screening for G6PD –deficiency in Isfahan, Iran: a quantitative assay.Journal of medical screening, 2008 vol. 15: (2) 62-64

Reclos GJ, Hatzidakis CJ, Schulpis KH. Glucose-6-phosphate dehydrogenase deficiency neonatal screening: preliminary evidence that a high percentage of partially deficient female neonates are missed during routine screening. J Med Screen. 2000;7:46–51.

Slusher TM, Vreman HJ, McLaren DW, et al. Glucose-6-phosphate dehydrogenase deficiency and carboxyhemoglobin concentrations associated with bilirubin-related morbidity and death in Nigerian infants. J Pediatr 1995;126:102–8

SukamalBisol, SumantaChakraborty, DipankarChattopadhyay, BiswajitBiswas, Sarbajit Ray. G6PD Screening of Babies born in a tertiary care hospital in west Bengal Pubmed2012:vol. 56: 2; 146- 148

Tanphaichitr U.S, Pung-amritt P, Yodthony S, Soongswany J, Mahasandana C, Suvatte V. G6PD-deficiency in the new born; its prevalence and relation of neonatal jaundice. South east Asian J Trop med public Health1995: 26 :(1); 37-41

Valaes T. Severe neonatal jaundice associated with glucose-6-phosphate dehydrogenase deficiency: pathogenesis and global epidemiology. ActaPaediatrSuppl 1994;394:58-76.

Yaish HM, Niazi GA, al Shaalan M, et al. Increased incidence of hyperbilirubinaemia in ‘unchallenged’ glucose-6-phosphate dehydrogenase deficiency in term Saudi newborns. Ann Trop Paediatr1991;11:259–66